|
KMID : 0545120040140051057
|
|
Journal of Microbiology and Biotechnology
2004 Volume.14 No. 5 p.1057 ~ p.1062
|
|
|
Characterization of Bacillus thuringiensis Having Insecticidal Effects Against Larvae of Musca domestica
|
|
Oh ST
Kim JK/Yang SY/Song MD
|
|
|
Abstract
|
|
|
|
The entomopathogenic bacterium Bacillus thuringiensis is the most widely used biopesticide. Insecticidal proteins coded by genes located in plasmids form typical parasporal crystalline inclusions during sporulation. We isolated a Bacillus thuringiensis strain having insecticidal activity against larvae of the house fly (M. domestica) from the soils at a pig farm in Korea and named it Bacillus thuringiensis SM. The culture filtrate from Bacillus thuringiensis SM showed strong lethality (83.3%) against M. domestica larvae. The parasporal crystal is enclosed within the spores’ outermost envelope as determined by transmission electron microscopy and exhibited a bipyramidal form. The crystal proteins of strain SM consisted of five proteins with molecular weights of approximately ~130 ~80 ~68 ~42 and ~27 kDa on a 10% SDS-PAGE (major band a size characteristic of Cry protein). Examination of antibiotic resistance revealed that the strain SM showed multiple resistant. The strain SM had at least three different plasmids with sizes of 6.6 9.3 and 54 kb. Polymerase chain reactions (PCRs) revealed the presence of cry1 cry4A2 and cry11A1 genes in the strain SM. The cry1 gene profile of the strain SM appeared in the three respective products of 487 bp [cry1A(c)] 414 bp [cry1D] and 238 bp [cry1A(b)]. However the strain SM has not shown the cry4A2 and cry11A1 genes. In in vivo toxicity assays the strain SM showed high toxicity on fly larvae (M. domestic) [with LC50 of 4.2 mg/ml LC90 of 8.2 mg/ml].
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|